Dear scientists, at the moment I am writing up my PhD thesis. In parallel I am looking for a new job outside the lab. I had a lot of fun during my PhD and it was a great and inter …

I am trying to run a plant extract in a native PAGE but the sample doesn't run, if run a little bit it gives a protein smear. Does anybody knows how can I solve the problem?

how can I remove a myc-tag from my overexpressed protein?

hello everyone! A question about PCR puzzled me many days! I transfected several kinds of siRNA to my tumor cell with lipofectamine. and then testify the transfection result with …

Well, obviously choosing a suitable polymerase for second strand ligation depends on a whole lot of experimental parameters. In my case the most crucial parameter is the efficient …

hello all, I am doing GST pull down experiment, where my protein tagged with N-terminal GST. and its purified and desalted. when i am doing interaction using Cell lysate (Hela o …

Hi, I am just wandering if it is possible to do nested PCR with the same primer used primarily? would appreciate suggestion on this matter.

Hi everyone! I started working in my current lab 5 years ago. I started with one box in the refrigerator and one in the freezer... Easy to keep track of things. But today, I have …

Hi I express my recombinant protein, with H5 insect cells, directly in the medium (Express Five). During my IMAC purification from the medium, I get a contaminant most likely Ferr …

Dear all, does anyone has experience of how long I have to incubate a protein sample with trypsin for a complete digestion? I incubated o.n. at 37°C. Is this generally enough time …

Our lab has an Äkta-based chromatography system and we are looking into using a silica-based gel filtration column. Specifically, we are hoping to use one of the BioSep range of co …

Hi all, I am currently attempting to crystallise a two domain protein which contains several structurally important disulfides. We have a high resolution structure of one domain ( …

I recently asked about the state of Macs in crystallography, and now I thought I'd ask about the state of Windows PCs. Windows 7 looks to be pretty good. I know that CCP4, Coot, …

I thought it might be valuable to have a list of blogs that people find useful. If you're a registered user on MajorGroove, feel free to update this list directly by clicking edit …

Hi everybody, I just crystallized a new project protein. How can I find a possible model for using molecular replacement? I have the sequence of my protein. Is it enough to make a …