Hello, I'm looking for a Phe auxotrophic strain of E Coli that can be used with plasmids with T7 promoter. Preferably BL21 (DE3). Can anybody direct me to a bank, lab or where to buy it? Thnxs.
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Have you tried one of these hits: http://www.google.de/search?q=phe+auxotrophic |
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I'm not sure if there is a pre-existing Phe auxotrophic with the λDE3 phage lysogenised that is commercially available. Your best bet is probably to source a regular Phe auxotrophic strain and then use a lysogenization kit to integrate the λDE3 phage into it. Novagen (Merck) sell a suitable kit, details can be found here: http://bit.ly/aNSEEf . The procedure is relatively straightforward. From that web page:
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