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Our lab has an Äkta-based chromatography system and we are looking into using a silica-based gel filtration column. Specifically, we are hoping to use one of the BioSep range of columns from Phenomenex, detailed here. Does anyone have any experience with these columns and would be able to answer: 1. If these columns are compatible with Äkta systems? 2. If any adapters are required? and 3. If there are any other silica-based gel filtration columns to consider? |
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Dear Roger, as long as you find a way to connect the Äkta's tubings to your desired colum, there should be no mechanical problem. The adaptors supplied with your Äkta provide lots of possibilities to play with. The other (i.e. crucial) aspect is, if Äkta can supply enough pressure/flow to operate these columns. I recommend to ask the supplier at what pressure they are operated. Good luck! Wo |
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