Tagged Questions

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votes
1answer
30 views

Which HRP Grade for Antibody Labeling?

Dear Experts, I would like to label an antibody with peroxidase by the periodate method. Which HRP grade / isoforms / purity should I use? Is there a practical advantage (or even …
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2answers
758 views

How to improve antibody binding to protein G sepharose beads?

I am immunoprecipitating my protein of interest from whole-cell lysate, using monoclonal mouse antibody bound and crosslinked to protein G sepharose beads. Many protocols claim tha …
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0answers
42 views

camKII or other pyramidal cell antibody for fixed frozen mouse brain sections

What is a good antibody and staining protocol for camKII? Is there another marker for pyramidal cells that has a good antibody? I am staining mouse brain, fixed frozen tissue. I …
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vote
0answers
8 views

Prediction Tool for Antigenic Peptides?

Dear Experts, I plan to order some custom peptide antibodies. While I have found a useful resource to estimate the antigenicity of peptide sequences, Antheprot (http://antheprot-p …