0
votes
1answer
30 views
Which HRP Grade for Antibody Labeling?
Dear Experts,
I would like to label an antibody with peroxidase by the periodate
method. Which HRP grade / isoforms / purity should I use? Is there a
practical advantage (or even …
2
votes
2answers
758 views
How to improve antibody binding to protein G sepharose beads?
I am immunoprecipitating my protein of interest from whole-cell lysate, using monoclonal mouse antibody bound and crosslinked to protein G sepharose beads. Many protocols claim tha …
0
votes
0answers
42 views
camKII or other pyramidal cell antibody for fixed frozen mouse brain sections
What is a good antibody and staining protocol for camKII? Is there another marker for pyramidal cells that has a good antibody? I am staining mouse brain, fixed frozen tissue. I …
1
vote
0answers
8 views
Prediction Tool for Antigenic Peptides?
Dear Experts,
I plan to order some custom peptide antibodies. While I have found a useful resource to estimate the antigenicity of peptide sequences, Antheprot (http://antheprot-p …