I am trying to run a plant extract in a native PAGE but the sample doesn't run, if run a little bit it gives a protein smear. Does anybody knows how can I solve the problem?

Dear all, does anyone has experience of how long I have to incubate a protein sample with trypsin for a complete digestion? I incubated o.n. at 37°C. Is this generally enough time …

Dear Experts, I would like to label an antibody with peroxidase by the periodate method. Which HRP grade / isoforms / purity should I use? Is there a practical advantage (or even …

Hi all, I need to make lipid micelles for an experiment. My lipids are dissolved in chloroform. I dry them under argon, resuspend in aqueous buffer (10mM sodium phosphate, 100mM K …