hello all, I am doing GST pull down experiment, where my protein tagged with N-terminal GST. and its purified and desalted. when i am doing interaction using Cell lysate (Hela o …

I'm not getting any positive cells in my titers. I'm using 293T, lipofectamine, and a Nature protocol with the DNA described therin (a GFP reporter). (Paper: Retrovirus-mediated s …

I am working with human fibroblast cells, specifically Det 551, and I am finding them to be really slow growing. Does anyone have a suggestion as to other normal human fibroblasts …