0
votes
2answers
135 views
Source for Phe Auxotrophic E. Coli BL21(DE3)?
Hello, I'm looking for a Phe auxotrophic strain of E Coli that can be
used with plasmids with T7 promoter. Preferably BL21 (DE3). Can
anybody direct me to a bank, lab or where to b …
0
votes
0answers
30 views
Size limit for subcloning into the MLS region in M13 helper phage?
Hi,
I have a question about isolating circular ssDNA from plasmids
carrying the f1 phage replication origin - does anyone know if there
is a size limit for the insert that can be …
0
votes
0answers
10 views
How to clone a Zinc finger region into Zinc Finger Nuclease?
If I know the Zinc finger amino acid sequence (total 100-150 aa including 4 fingers and sequence between them), how can I clone (in-frame fused) this sequence to a ZFN vector which …