Tagged Questions

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Source for Phe Auxotrophic E. Coli BL21(DE3)?

Hello, I'm looking for a Phe auxotrophic strain of E Coli that can be used with plasmids with T7 promoter. Preferably BL21 (DE3). Can anybody direct me to a bank, lab or where to b …
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0answers
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Size limit for subcloning into the MLS region in M13 helper phage?

Hi, I have a question about isolating circular ssDNA from plasmids carrying the f1 phage replication origin - does anyone know if there is a size limit for the insert that can be …
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0answers
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How to clone a Zinc finger region into Zinc Finger Nuclease?

If I know the Zinc finger amino acid sequence (total 100-150 aa including 4 fingers and sequence between them), how can I clone (in-frame fused) this sequence to a ZFN vector which …