Hi all, I am currently attempting to crystallise a two domain protein which contains several structurally important disulfides. We have a high resolution structure of one domain ( …

I recently asked about the state of Macs in crystallography, and now I thought I'd ask about the state of Windows PCs. Windows 7 looks to be pretty good. I know that CCP4, Coot, …

Hi everybody, I just crystallized a new project protein. How can I find a possible model for using molecular replacement? I have the sequence of my protein. Is it enough to make a …

Are Linux workstations still required these days to do crystallography, or can everything be done on a Mac? While roughly twice as expensive, perhaps Macs are more useful as they …

I'm aware of GPU computing making great strides in tomography, but are there any projects which use GPGPUs in structural biology? Or, are the algorithms from, for example, crystal …

Howdy, One of our users was trying to re-run some Refmac jobs originally run with version 5.5.0088 under version 5.5.0092, and he noticed that although the logfile shows Refmac co …

I have been trying to fit the ligand in electron density since a long time. I could place it in the right place by simply using 'rotate/translate zone'. Now i am facing difficulty …

We are thinking of buying a dynamic/static light scattering unit to analyze our samples. The Nanostar from Wyatt (previously from Dynapro) seems to combine both DLS and static ligh …

What order do you recommend reading crystallography books? Two parts: 1) Suggestion a book if it is not listed 2) Vote up books that should be read first (are a good starting po …

When calculating rmsd values, are some methods more rigorous/correct than others? I have used super and align in PyMOL and just taken the readout from the terminal window, but eac …

I have quite thin needle crystals. They break or bend when I try to mount and freeze them in small loops. If I use larger loops, the solvent in the loop causes a lot of background …

Does anyone know if the program MODIP is still available? Anyone know any other programs that will do what MODIP does? Thanks! Mark

I have a question regarding adding a ligand to my model. I'm not quite sure how to do it. I have obtained the ligand in two different ways, either in SMILES format from the drugban …

I am trying to assemble my model (after molecular replacement and automatic rebuilding) from smaller parts of different models. To avoid hand editing of pdb files I tried the "copy …

Can anybody recommend a program to plot a couple of amino acid residues of a active site/cavity in 2D? A screenshot of the active site is to confusing. Thanks, P. edit: As far as …