Well, obviously choosing a suitable polymerase for second strand ligation depends on a whole lot of experimental parameters. In my case the most crucial parameter is the efficient …

hello all, I am doing GST pull down experiment, where my protein tagged with N-terminal GST. and its purified and desalted. when i am doing interaction using Cell lysate (Hela o …

Dear all, does anyone has experience of how long I have to incubate a protein sample with trypsin for a complete digestion? I incubated o.n. at 37°C. Is this generally enough time …

Hello, I'm looking for a Phe auxotrophic strain of E Coli that can be used with plasmids with T7 promoter. Preferably BL21 (DE3). Can anybody direct me to a bank, lab or where to b …

Hi all. I seem to have a serious problem with primer dimer formation. I am not sure but I believe it is for this reason that my PCRs aren't working lately. Is there anything I can …

I have been trying to design primers for the spice variants. How can I design exon specific primers to identify the spice variants? There is no information on the number of exons i …

Hi everybody, I just asked myself what kinds of analysis is possible for a protein structure.I know that there are so many programms and software for almost any type of question a …

I want to know how to design RT-qPCR primers that are specific enough to measure the expression of one splice variant and not others? Any ideas? Thanks.

I would like to attempt lentiviral or retroviral transduction of my gene of interest into primary murine splenic B cells. I have used both viral systems successfully on B cell line …

Does anyone know of a C-terminal sequencing service for proteins? Preferably one where one can simply send a blot to by normal mail. Apparently, here in Spain, there is none. Gree …

I have a question about the Em7 promoter. I am working on a BAC construct at the moment, which I would use for generating a transgenic line. I have already made the targeting vecto …