Tagged Questions

0
votes
1answer
17 views

the expression of negative control is always higher than that of positive control,why?

hello everyone! A question about PCR puzzled me many days! I transfected several kinds of siRNA to my tumor cell with lipofectamine. and then testify the transfection result with …
2
votes
4answers
191 views

PCR primer dimer formation

Hi all. I seem to have a serious problem with primer dimer formation. I am not sure but I believe it is for this reason that my PCRs aren't working lately. Is there anything I can …
0
votes
2answers
188 views

Abbreviations for all types of PCR

We should agree upon what tags to use when discussing all variations of PCR on MajorGroove. PCR abbreviations can be confusing to the non-specialist. Although RT always means "re …