how can I remove a myc-tag from my overexpressed protein?

Hi I express my recombinant protein, with H5 insect cells, directly in the medium (Express Five). During my IMAC purification from the medium, I get a contaminant most likely Ferr …

Our lab has an Äkta-based chromatography system and we are looking into using a silica-based gel filtration column. Specifically, we are hoping to use one of the BioSep range of co …

I would like to use the HRV 3C protease. TEV is not really an option since it works with dtt and my protein is full of disulfide bridges. So i would like to know if you have any …

I am immunoprecipitating my protein of interest from whole-cell lysate, using monoclonal mouse antibody bound and crosslinked to protein G sepharose beads. Many protocols claim tha …

Dear experts, I try to purify a chicken IgY by affinity chromatography. The hapten (a small "chemical" molecule, not a protein or peptide) is covalently coupled to sepharose. In …

My protein is relatively pure except the dimer. i used 10 mM TCEP to reduce it (directly added 1 M TCEP to make final volume of 10mM and kept at room temperature for 10 mins] then …

I am now preparing to label my protein with selenium-methionine for phasing. As I have heard that selenium-methionine is prone to oxidation, so is it a must to add reducing agent a …

Our protein is stable in acidic pH at a pI of 6. We do not have any tag on it, and it is expressed in inclusion body form. Which ion exchange chromatography will suit it, cation or …

I purified my protein under acidic conditions pH-4 with the reason that it will be free from DNA. Even though its 280 absorption dominates the 260 absorption the 280/260 ratio is a …

Hi all, I'm looking at getting some nickel magnetic beads. I've never used them before, and they seem pretty expensive. Does anyone know of a good inexpensive supplier? Thanks in …

Dear All, We are currently setting up a fermentation core and are considering purchasing a T1 Sharples continuous flow centrifuge. It is a mid-range capacity instrument. We will b …

We perform refolding of our protein by diluting it 1:10 fold, but we get a low yield. I have studied pulsatile refolding in the book "Therapeutic proteins: methods and protocols". …