0
votes
4answers
53 views
nested PCR, rt-PCR
Hi,
I am just wandering if it is possible to do nested PCR with the same primer used primarily?
would appreciate suggestion on this matter.
0
votes
3answers
100 views
Primer design for splice variants
I have been trying to design primers for the spice variants. How can I design exon specific primers to identify the spice variants? There is no information on the number of exons i …
0
votes
2answers
188 views
Abbreviations for all types of PCR
We should agree upon what tags to use when discussing all variations of PCR on MajorGroove. PCR abbreviations can be confusing to the non-specialist. Although RT always means "re …
1
vote
2answers
115 views
Protocol for single C. elegans RT-PCR?
Dear colleagues,
I'm advised that putative positives from RNA-interference (RNAi) experiments (using feeding strains from Geneservice {Fraser et al [2000]. Nature 408 (6810) 325-3 …
