Hi all, I'm using the distributed Refmac version 5.5.0109 (OS = Centos 4.6) and I get this cryptic error message at the end of cycle 13: 1 0.3931933 1.013090 …

In proteins (and RNA), the term "rotamer" is used to refer to a specific conformer (or class of conformers), found in structures. According to IUPAC (http://goldbook.iupac.org/R054 …

We are thinking of buying a dynamic/static light scattering unit to analyze our samples. The Nanostar from Wyatt (previously from Dynapro) seems to combine both DLS and static ligh …

I'm aware of GPU computing making great strides in tomography, but are there any projects which use GPGPUs in structural biology? Or, are the algorithms from, for example, crystal …

Dear experts, I try to purify a chicken IgY by affinity chromatography. The hapten (a small "chemical" molecule, not a protein or peptide) is covalently coupled to sepharose. In …

Howdy, One of our users was trying to re-run some Refmac jobs originally run with version 5.5.0088 under version 5.5.0092, and he noticed that although the logfile shows Refmac co …

I thought it might be valuable to have a list of blogs that people find useful. If you're a registered user on MajorGroove, feel free to update this list directly by clicking edit …

Well, obviously choosing a suitable polymerase for second strand ligation depends on a whole lot of experimental parameters. In my case the most crucial parameter is the efficient …

I am trying to soak some sugars in my crystals, but the cystallization condition has a pH of 4.0. Does anybody has any experience with acidic oxidation in such a case. I think I ca …

Dear All, We are currently setting up a fermentation core and are considering purchasing a T1 Sharples continuous flow centrifuge. It is a mid-range capacity instrument. We will b …

We perform refolding of our protein by diluting it 1:10 fold, but we get a low yield. I have studied pulsatile refolding in the book "Therapeutic proteins: methods and protocols". …

Dear scientists, at the moment I am writing up my PhD thesis. In parallel I am looking for a new job outside the lab. I had a lot of fun during my PhD and it was a great and inter …

Does anyone know if the program MODIP is still available? Anyone know any other programs that will do what MODIP does? Thanks! Mark

Dear Experts, I plan to order some custom peptide antibodies. While I have found a useful resource to estimate the antigenicity of peptide sequences, Antheprot (http://antheprot-p …

I have a question about the Em7 promoter. I am working on a BAC construct at the moment, which I would use for generating a transgenic line. I have already made the targeting vecto …